Journal: bioRxiv

Article Title: UVB-Induced Genotoxic Stress Activates the DNA Damage Response and Innate Immune Pathways in Sea Urchin Coelomocytes

doi: 10.64898/2026.01.14.699502

Figure Lengend Snippet: (a) Viability of S. purpuratus coelomocytes and human peripheral blood mononuclear cells (PBMCs) measured 24 h after exposure to a range of UVB doses. For coelomocytes, mean values ± the standard deviation of biological triplicates (n = 3) are shown. For PBMCs, mean values ± the standard deviation of technical triplicates are shown. Individual points are overlaid as white circles. (b) Example of a comet with the head and tail region indicated. (c) Average tail DNA percent and (d) average percent of damaged cells (the percent of cells with any amount of DNA damage) in S. purpuratus coelomocytes challenged with 1,000 mJ/cm 2 UVB at 1, 6, and 24 h post-exposure (red bars) versus control, untreated coelomocytes (purple bars). Error bars are mean values ± the standard deviation of biological triplicates (n = 3). Individual points are overlaid as white circles. Significant differences between control and UVB-treated cells were analyzed using a one-way ANOVA and post-hoc TukeyHSD test (***, adjusted p value < 1 x 10 -3 ).

Article Snippet: In contrast, human peripheral blood mononuclear cells (PBMCs; ATCC PCS-800-011) demonstrated a sharp decline in viability following exposure to UVB with an LD 50 value of 871 mJ/cm 2 .

Techniques: Standard Deviation, Control

Journal: Frontiers in Immunology

Article Title: Trispecific eFab-eIg T-cell engagers targeting HER2 and HER3

doi: 10.3389/fimmu.2025.1642454

Figure Lengend Snippet: Target cell binding and cytotoxicity. (A) Binding of different eIg molecules (eFab-eIg HER2xHER3xCD3; eIg HER2xCD3; eIg HER3xCD3) to BT474, LIM1215 and MDA-MB-468 cells was analyzed via flow cytometry. (B) Killing of target cells (BT474, LIM1215 and MDA-MB-468) incubated with bi- or trispecific eIg molecules and PBMCs (donor: HN#7 for BT474; HN#6 for LIM1215 and AH#1 for MDA-MB-468) at an effector-to-target (E:T) ratio of 10:1 for 3 days. Mean of duplicates ± SD, n=1.

Article Snippet: Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coat of healthy donors (Klinikum Stuttgart/Institut für Klinische Transfusionsmedizin und Immungenetik Ulm gemeinnützige GmbH, Germany) by Ficoll density gradient centrifugation (Lymphocyte Separation Medium 1077, Promocell, C-44010) and cultivated in RPMI 1640, 10% FCS.

Techniques: Binding Assay, Flow Cytometry, Incubation

Journal: Frontiers in Immunology

Article Title: Trispecific eFab-eIg T-cell engagers targeting HER2 and HER3

doi: 10.3389/fimmu.2025.1642454

Figure Lengend Snippet: T-cell activation by TCEs. Release of IL-2, IL-6 and TNFα after 24 h and IFNγ after 48 h by PBMCs co-cultured with LIM1215 using an effector-to-target ratio of 10:1 analyzed by sandwich ELISA. Mean ± SD, n=2 - two individual donors).

Article Snippet: Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coat of healthy donors (Klinikum Stuttgart/Institut für Klinische Transfusionsmedizin und Immungenetik Ulm gemeinnützige GmbH, Germany) by Ficoll density gradient centrifugation (Lymphocyte Separation Medium 1077, Promocell, C-44010) and cultivated in RPMI 1640, 10% FCS.

Techniques: Activation Assay, Cell Culture, Sandwich ELISA

Journal: Frontiers in Immunology

Article Title: Trispecific eFab-eIg T-cell engagers targeting HER2 and HER3

doi: 10.3389/fimmu.2025.1642454

Figure Lengend Snippet: Killing of BT474 spheroids. BT474 spheroids with a diameter of approximately 250 µm were used as target cells and incubated with different amount of trispecific eFab-eIg antibodies as well as different number of PBMCs (donor: AL#1). In addition, the induction of apoptosis was analyzed using propidium iodide (PI; 1 µg/mL). Cells were incubated with antibodies and the PBMCs for 48 h at 37°C. n=1.

Article Snippet: Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coat of healthy donors (Klinikum Stuttgart/Institut für Klinische Transfusionsmedizin und Immungenetik Ulm gemeinnützige GmbH, Germany) by Ficoll density gradient centrifugation (Lymphocyte Separation Medium 1077, Promocell, C-44010) and cultivated in RPMI 1640, 10% FCS.

Techniques: Incubation